233 fb 025 media 3 (R&D Systems)

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R&D Systems 233 fb 025 media 3
233 Fb 025 Media 3, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 890 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/233+fb+025/Recombinant+Human+FGF+basic%2FFGF2%2FbFGF+(146+aa)+Protein/bio_rxiv__64898__2026__01__13__699271-146-102-100
Average 96 stars, based on 890 article reviews

233 fb 025 media 3 - by Bioz Stars, 2026-09

96/100 stars

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Article Title: Single-cell atlas of major haematopoietic tissues sheds light on blood cell formation from embryonic endothelium
Article Snippet: .. On the day of sorting, existing OP9 medium was replaced with rich hemogenic endothelium mix, consisting of IMDM (Lonza BE12-726F); 10% fetal bovine serum (Gibco, #10270-42G9552K); 1% L-glutamine (Gibco, #25-030-024); human holotransferrin 0.6% (Roche, 10652202001); monothioglycerol 0.0039 % (Sigma, M6145); 25μg/ml L-ascorbic acid (Sigma, A4544); 0.0024 % of LIF 1 mg/ml (EMBL Heidelberg); 0.5% of recombinant murine SCF 10μg/ml (Peprotech, #250-03); 0.1% of recombinant murine IL-3 25 μg/ml (Peprotech, #213-13); 0.04% of recombinant murine IL-11 12.5μg/ml (Peprotech, #220-11); 0.1% of recombinant murine IL-6 10μg/ml (Peprotech #216-16); 0.1% of recombinant mouse Oncostatin M 10μg/ml (R&D Systems, #495-MO-025); 0.01% of recombinant human FGF basic protein 10μg/ml (R&D Systems, #233-FB-025). .. The E11.5 Yolk sacs from miR144/451 +/GFP mouse embryos were first collected in 1xPBS+10% Fetal Bovine Serum (10270106, Gibco).

Article Title: Id4 Downstream of Notch2 Maintains Neural Stem Cell Quiescence in the Adult Hippocampus.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies BrdU AbD Serotec/Bio-Rad Cat #OBT0030; RRID:AB_609568 Doublecortin Santa Cruz Cat #sc-8066 (C-18); RRID:AB_2088494 DS-red/mCherry/Tomato Clontech Takara Cat #632496; RRID:AB_10013483 GFAP (Glial Fibrillary acidic protein) Sigma Cat #G3893; RRID:AB_477010 GFAP (Glial Fibrillary acidic protein) Sigma Cat #G9269; RRID:AB_477035 GFAP (Glial Fibrillary acidic protein) Santa Cruz Cat #sc-6170 (C-19); RRID:AB_641021 GFP (Green fluorescent protein) Aves labs Cat #GFP-1020 AB_10000240 GFP (Green fluorescent protein) Invitrogen Cat #A11122; RRID:AB_221569 GFP (Green fluorescent protein) AbD Serotec/Bio-Rad Cat #4745-1051; RRID:AB_619712 Id4 Biocheck Cat #BCH-9/82-12 Ki67 Abcam Cat #ab15580; RRID:AB_443209 Mash1 (Ascl1) PharMingen Cat #556604; RRID:AB_396479 anti-Myc tag Abcam Cat #ab9132; RRID:AB_307033 Nestin RAT401 Millipore Cat #MAB353; RRID:AB_94911 NeuN Millipore Cat #MAB377; RRID:AB_2298772 Notch1 H. Robson MacDonald ISREC, Lausanne Anti-Notch1(Rat) Notch2 H. Robson MacDonald ISREC, Lausanne Anti-Notch2 (Rat) PCNA (proliferating cell nuclear antigen) DAKO Cat #M0879; RRID:AB_2160651 S100b Dako Cat #Z0311; RRID:AB_10013383 Sox2 Santa Cruz Cat #sc-17320; RRID:AB_2286684 Sox10 Santa Cruz Cat #sc-17342; RRID:AB_2195374 Tbr2 eBioscience Cat #14-4875-82; RRID:AB_11042577 donkey anti-mouse Cy3 Jackson ImmunoResearch Cat #715-165-151; RRID:AB_2315777 donkey anti-mouse Alexa488 Jackson ImmunoResearch Cat #715-546-151; RRID:AB_2340850 donkey anti-mouse Alexa647 Jackson ImmunoResearch Cat #715-605-150; RRID:AB_2340862 donkey anti-rat Cy3 Jackson ImmunoResearch Cat #712-166-153; RRID:AB_2340669 donkey anti-rabbit Alexa488 Jackson ImmunoResearch Cat #711-545-152; RRID:AB_2313584 donkey anti-chicken Alexa 488 Jackson ImmunoResearch Cat #703-545-155; RRID:AB_2340375 donkey anti-goat Alexa647 Jackson ImmunoResearch Cat #705-605-147; RRID:AB_2340437 donkey anti-sheep Alexa 488 Jackson ImmunoResearch Cat #713-545-147; RRID:AB_2340745 Bacterial and Virus Strains Adenovirus pAd/PLGFAPp-NLSCre-pA Merkle et al., 2007 https://doi.org/10.1126/science.1144914 Adenovirus Adeno-Cre Nyfeler et al., 2005 https://doi.org/10.1038/sj.emboj.7600816 Retrovirus pMI-NLSCre This paper Retro-Cre Lentivirus pLVX-IRES-tRFP This paper Lenti-tRFP Lentivirus pLVX-Id4-IRES-tRFP This paper Lenti-Id4-IRES-tRFP Chemicals B27 Supplement (50X) GIBCO Cat #17504044 BrdU Sigma Cat #B9285 DMEM:F-12 +GlutaMAX GIBCO Cat # 31331-028 EGF (Recombinant Mouse EGF Protein, CF) R&D Systems Cat #MAB2028-100 FGF2 (Recombinant Mouse FGF basic, CF) R&D Systems Cat #233-FB-025 (Continued on next page) Cell Reports 28, 1485–1498.e1–e6, August 6, 2019 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Formaldehyde (16% methanol free) Thermo Fisher Scientific Cat #28906 Glycine Sigma Cat #50046-1KG L-15 Medium Invitrogen Cat #31415029 Normal Donkey Serum Jackson ImmunoResearch Cat #017-000-121 Papain Sigma Cat #P3125-100MG Power SYBR Green PCR Master Mix Thermo Fisher Scientific Cat #4367659 Protein G Dynabeads Invitrogen Cat #10003D Tamoxifen Sigma Cat #T5648-1G Trizol Invitrogen Cat #VX15596026 Trypsin inhibitor Glycine max (Soybean)/Ovomucoid Sigma Cat #T6522-100MG Critical Commercial Assays RNeasy Mini Kit QIAGEN Cat #74104 REPLI-g WTA Single Cell Kit QIAGEN Cat #150063 SuperScript IV VILO Master Mix with ezDNase Enzyme Thermo Fisher Scientific Cat #11766500 Click-iT EdU Alexa Fluor 647 Imaging Kit Life Technologies Cat #C10340 Experimental Models: Cell Lines Mouse: adult hippocampal Rosa26R-CAG::GFP neural stem cell line This paper DG NSC CAG Mouse: adult hippocampal Rosa26R-CAG:: floxed-STOP-Notch2ICD Rosa26R-CAG::GFP neural stem cell line This paper DG NSC N2ICD Experimental Models: Organisms/Strains Mouse: BL6J:Notch2lox/lox Besseyrias et al., 2007 Notch2tm1Frad Mouse: BL6J:Rosa26R-CAG::GFP Tchorz et al., 2012 https://doi.org/10.1371/journal.pone.0030011 Mouse: BL6J:Hes::CreERT2 Lugert et al., 2010 Tg(Hes5-cre/ERT2)2Vtlr Mouse: Notch2::CreERT2-SAT Fre et al., 2011 https://doi.org/10.1371/journal.pone.0025785 Mouse: Rosa26R-CAG::tdTomato The Jackson Laboratory Gt(ROSA)26Sortm9(CAG-tdTomato)Hze Mouse: BL6J:Hes5::GFP Basak and Taylor, 2007 Tg(Hes5-EGFP)2Vtlr Mouse: BL6J:BLBP::mCherry Giachino et al., 2014 Tg(BLBP-mCherry)2Vtlr Mouse: BL6J:Rosa26R-CAG::floxed-STOPNotch2ICD Tchortz et al., 2009 https://doi.org/10.1002/hep.23048 Mouse: Id4lox/lox Best et al., 2014 https://doi.org/10.1242/dev.108498 Oligonucleotides and Recombinant DNA qRT-PCR and ChIP primers This paper Table S2 Plasmid: pCAGGS-mCherry Gurtan et al., 2012 RRID: Addgene_41583 Plasmid: pMI-NLSCre Verdon Taylor Lab pMI-NLSCre Plasmid: pLVX-IRES-tRFP This paper pLVX-IRES-tRFP Plasmid: pLVX-Id4-IRES-tRFP This paper pLVX-Id4-IRES-tRFP Plasmid: pCMV-VSV-G Stewart et al., 2003 RRID: Addgene_8454 Plasmid: psPAX2 (Didier Trono Lab) RRID: Addgene_12260 Antisense LNA GapmeR-Id4 Exiqon Product #300600 Antisense LNA GapmeR-Negative control A Exiqon Product #300610 Software and Algorithms GraphPad Prism 7 GraphPad https://www.graphpad.com/scientificsoftware/prism/ FIJI (ImageJ v1.0) Schindelin et al., 2012 http://fiji.sc Photoshop Adobe https://www.adobe.com/Photoshop DAVID Bioinformatics Resource v6.7 Huang da et al., 2009 https://david.ncifcrf.gov Bowtie2 Langmead and Salzberg, 2012 https://doi.org/10.1038/nmeth.1923 (Continued on next page) e2 Cell Reports 28, 1485–1498.e1–e6, August 6, 2019

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